can a router plug plug into Port #2 into on a 2 port ION?

I was using  a hidden network via a router. IThe router was plugged into port #1 and the network into Port #2. The router was unplugged from the ION. The network cable was then plugged into port 1. Can the router be plugged into port #2?  Does the network cable need to be in Port #1?

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